• Associate Professor, Dept. of Biomedical & Molecular Sciences (DBMS)
• Cancer Biology & Genetics (CBG) division, Queen's Cancer Research Institute
• Graduate students in my lab enrol in Biochemistry graduate program
  PDF applicants are members of DBMS & CBG
• Applications are welcome and should include your CV and statement of interests.

Recently, a new type of phosphoinositide-binding module has been identified (F-BAR) in the Pombe Cdc15 Homology (PCH) family of proteins (that includes the Fer/Fes kinases).  We are studying F-BAR domain functions in both the Fer/Fes kinases, as well as in adaptor proteins (CIP4 & Toca-1) that interact with Cdc42 and its effector N-WASP.  F-BAR adaptor proteins function promoting membrane invaginations through coordinated actions of their F-BAR and SH3 domains.  F-BAR domains bind membranes and induce curvature, while SH3 domain interactions recruit N-WASP (promoting actin assembly that constricts the neck) and Dynamin (that pinches off the nascent vesicle).  The F-BAR kinases may cooperate with F-BAR adaptors via phosphorylation of actin regulatory proteins (cortactin and HS1) that stabilize branched filamentous actin (F-actin).  F-BAR proteins have been implicated in contributing to inflammatory disorders, immunity and cancer.  Our lab is actively pursuing how F-BAR proteins regulate cancer progression and metastases using cell-based assays and animal models.

Andrew W.B. Craig (2011) FES/FER kinase signaling in hematopoetic cells and leukemias. Frontiers in Bioscience, in press.

^{Jinghui Hu, Alka Mukhopadhyay, Peter Truesdell, UK Mukhopahyay, Alan Mak and Andrew WB Craig (2011)  Cdc42-Interacting Protein 4 is a Src substrate that regulates invadopodia and breast tumor invasiveness via promoting MT1-MMP endocytosis, J. Cell Sci., 124: 1739-1751.}

^{Jinghui Hu, Alka Mukhopadhyay and Andrew WB Craig (2011) Transducer Of Cdc42-dependent actin assembly promotes epidermal growth factor-induced cell motility and invasiveness, J. Biol. Chem., 286:2261-72.}

^{Julie Smith, Lionel Samayawardhena and Andrew Craig (2010) Fps/Fes protein-tyrosine kinase regulates mast cell adhesion and migration downstream of Kit and ƒÒ1 integrin receptors, Cell. Signal, 22:427-436.}

^{Victor A. McPherson,  Namit Sharma, Stephanie Everingham, Julie Smith, Helen H. Zhu, Gen-Sheng Feng and Andrew W.B. Craig (2009) SHP2 Protein-Tyrosine Phosphatase Promotes Fc£`RI-induced Activation of Fyn and Erk Pathways Leading to TNF£\ Release from Bone Marrow-derived Mast Cells, J. Immunol., 183:4940-4947.}

^{Jinghui Hu, Flavia Troglio , Alka Mukhopadhyay, Stephanie Everingham, Ester Kwok, Giorgio Scita, and Andrew W.B. Craig (2009) F-BAR-containing adaptor CIP4 localizes to early endosomes and regulates Epidermal Growth Factor Receptor trafficking and downregulation, Cell. Signal, 21, 1686-1697.}

^{Victor McPherson, Stephanie Everingham, Robert Karisch, Julie Smith, Chris Udell, Jimin Zheng, Zongchao Jia, and Andrew W.B. Craig (2009) Contributions of F-BAR and SH2 Domains of FES Protein-Tyrosine Kinase For Coupling to the FcƒÕRI Pathway in Mast Cells, Mol. Cell. Biol. 29:389-401.}

^{Lei  Liu, Andrew W. Craig, Heather D. Meldrum, Bruce E. Elliott, and Marlys L. Koschinsky (2008) Integrin a_Vb₃ is Required for Apolipoprotein(a) Stimulation of Vascular Endothelial Cell Growth and Migration, Biochem. J., 418:325-36.}

^{Lionel A. Samayawardhena, Reuben Kapur and Andrew W.B. Craig (2007) Involvement of Fyn kinase in c-Kit and integrin-mediated Rac activation, cytoskeletal reorganization and chemotaxis of mast cells, Blood, 109:3679-3686.}

^{C.M. Udell, L.A. Samayawardhena, Y. Kawakami, T. Kawakami, and A.W.B. Craig (2006) Fer and Fps/Fes participate in a Lyn-dependent pathway from Fc£`RI to Platelet-Endothelial Cell Adhesion Molecule-1 to limit mast cell activation, J. Biol. Chem., 281:20949-20957.}

^{L. Samayawardhena, J. Hu, P. Stein, and A.W.B. Craig (2006) Fyn kinase acts upstream of Shp2 and p38 mitogen-activated protein kinase to promote chemotaxis of mast cells towards stem cell factor, Cell. Signal, 18: 1447-54.}

^{W. Qi, K.V.J. Ebbert, A.W.B. Craig, P.A. Greer and D.M. McCafferty (2005) Absence of Fer protein-tyrosine kinase exacerbates endotoxin-induced intestinal epithelial barrier dysfunction in vivo, Gut, 54:1091-97.}

^{G. Xu, A.W.B. Craig, P. Greer, M. Miller, P.Z. Anastasiadis, J. Lilien and J. Balsamo (2004).  An Epigenetic Loop Regulating the Tyrosine Phosphorylation of Beta-Catenin and Cadherin Function.  J. Cell Sci,117:3207-3219.}

^{L. Fan, C. Di Ciano-Oliviera, S.A. Weed, A.W.B. Craig, P.A. Greer, O.D. Rotstein and A. Kapus (2004). Actin Depolymerization-Induced Tyrosine Phosphorylation of Cortactin: The Role of Fer Kinase. Biochem. J.,380:581-591.}

^{A. Vultur, J. Cao, R. Arulanandam, J. Turkson, R. Jove, P. Greer, A. Craig, B. Elliot and L. Raptis (2004) Cell to cell adhesion modulates Stat3 activity in normal and breast carcinoma cells. Oncogene, 23:2600-2616.}