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Tuesday 20 Feb/18 at 2:00 pm - a QCRI Frontiers of Cancer Research presentation by Dr. Douglas Richardson

Published Wed Feb 14th 2018

a QCRI Frontiers of Cancer Research presentation!
Douglas Richardson, PhD

Director of Imaging, Lecturer on Molecular & Cellular Biology

Harvard Center for Biological Imaging (Harvard University)

Tuesday 20 Feb/18 at 2:00 pm

QCRI Conference Rooms 100/101

“Measuring endosomal pH beyond the diffraction limit"

Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. One widely used application of biosensors is to monitor the acidification of vesicles during endocytosis, an essential function that aids in intracellular cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250 nm diffraction limit of light microscopy. Therefore, to aid in investigations of pH dynamics during endocytosis at the nanoscale, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STED nanoscopy.

Host: Dr. Lois Mulligan (mulligal@queensu.ca)

Everyone is welcome!