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QCRI FCR Seminar (Dr. Douglas Richardson)

  • Event Start: Feb 20, 2018 2:00 pm
    Location: QCRI Conference Rooms 100/01

    a Frontiers of Cancer Research presentation!

    Douglas Richardson, PhD
    Director of Imaing, Lecturer on Molecular and Cellular Biology
    Harvard Center for Biological Imagining (Harvard University)

    Tuesday 20 Feb/18 at 2:00 pm in QCRI 100/01 conference roms

    "Measuring endosomal pH beyond the diffraction limit"

    Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. One widely used application of biosensors is to monitor the acidification of vesicles during endocytosis, an essential function that aids in intracellular cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250 nm diffraction limit of light microscopy. Therefore, to aid in investigations of pH dynamics during endocytosis at the nanoscale, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STED nanoscopy.

    Host: Dr. Lois Mulligan (mulligal@queensu.ca)

    Everyone is welcome!